Other tools: Primer design

    Another feature available in the website is the possibility to use PRIMER3 to design primers graphically over any region of interest, including within any of the reference genomes.

    The first step is to select the primer design option from gbrowse and click on go. A new webpage will be shown with the position of interest indicating the region that is going to be flanked by the primers.

    As can be seen in the figure there is a red line within the CDS (blue horizontal line) indicating the point of interest. Then, we have to select the parameters for PRIMER3 in order to design our primers. We click on Design primer and the primer sets will be shown.